Applications of Ultraviolet-Visible Spectroscopy: The 2022 Guide

piątek, 27 maj 22, 17:05

In the laboratory, UV-visible spectroscopy is used to analyze the structure of organic molecules...

In the laboratory, UV-visible spectroscopy is used to analyze the structure of organic molecules. This technique can be used to determine how a substance absorbs light and how much energy it absorbs. UV-visible spectroscopy is also used to determine the amount of dissolved oxygen in the water.

The absorption spectrum of a molecule gives information on its electronic energy levels and molecular structure. In other words, it gives information about the way electrons are distributed within a molecule and whether these electrons are able to absorb radiation or not.

Therefore, if we know a molecule's absorption spectrum, we can conclude what type of atomic bonds it has and its chemical formula (the number of atoms in each molecule). In other words, UV-visible spectroscopy has applications in many fields including biochemistry, environmental science, and medicine.

Cuvettes are small glass or plastic containers with a cylindrical shape and a conical base. They are often used in laboratories for holding small quantities of liquid.

In UV-visible spectroscopy, uv cuvettes are used to contain the sample that is being analyzed. The cuvette is placed in the spectrophotometer, and the light passed through the sample is analyzed.

Spectrophotometers are instruments that measure the amount of light absorbed by a sample. They are used to measure the absorption spectrum of samples. The spectrophotometer used in this experiment is a double beam spectrophotometer.

It has two optical paths, one for sending light into the sample, and another for receiving it after it has passed through the sample.

The absorption spectrum of a sample can be either monochromatic or polychromatic. A monochromatic source gives a single wavelength of light at any given time, while a polychromatic source gives more than one wavelength at once (for example sunlight is polychromatic).

The cuvette holder can be a plastic cup or a glass beaker. A simple DIY cuvette holder is made by cutting a piece of plastic in half, bending it into a circular shape, and gluing the two halves together at the top.

The cuvette holder should contain enough room for both quartz and borosilicate glass cuvettes to fit inside (a small amount of room should be left between the cuvettes so that the edge of one is not touching the other).

Warm water is needed to melt quartz or borosilicate glass. You can use any type of water; tap water works well. However, you will need to boil it before use: Remove as many impurities and oxygen as possible from tap water by filtering it through layers of paper towels or cheesecloth.

The water is boiled in an open spot on a stovetop for about 20 minutes until it starts bubbling (when salt starts forming crystals on the bottom of the pot).

When boiling water, make sure that there are no flames in close proximity (this may cause your experiment to explode).

This can be done by placing something heavy on top of your pot while it's boiling; if you don't have anything heavy handy, you could use a metal bowl filled with sand or gravel.